TOTAL PHENOLS AND FREE RADICAL SCAVENGING CAPACITY OF FIVE SELECTED HERBS IN CANTILAN, SURIGAO DEL SUR

Gemma A. Gruyal

Abstract


Many plants possess antioxidant components that provide efficacy in lowering incidence and in lowering mortality rates of degenerative diseases in human. In this article, five selected herbs were evaluated for their total phenols using Folin-Ciocalteu (FC) reagent and gallic acid monohydrate as the standard. 1, 1-diphenyl-2-picrylhydrazyl (DPPH) assay was used to evaluate free radical scavenging capacity. Results showed that the phenolic content of each herbal plant extracts varied from 31.95 mg/g to 91.02 mg/g and their free radical scavenging capacity ranged from 32.82% to 93.48%. Correlation (p<0.05) between the phenolic content and the free scavenging capacity of the five selected herbal plants was observed.

Keywords


phenols, antioxidant, herbs, radical scavenger, 1,1-diphenyl-2-picryl hydrazyl

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References


Chan, E.W.C., Lim, Y.Y. & Chew, Y.L. (2007). Antioxidant activity of Camellia sinensis leaves and tea from a lowland plantation in Malaysia. Food Chemistry, 102(4), 1214-1222. doi:10.1016/j. foodchem.2006.07.009.

Chew,Y.L, Chan, E.W.L., Tan, P.L., Lim,Y.Y., Stanslas, J., & Goh, J.K. (2011). Assessment of phytochemical content, polyphenolic composition, antioxidant and antibacterial activities of leguminosae medicinal plants in Peninsular Malaysia. BMC Complementary & Alternative Medicine, 11, 12. doi:10.1186/1472- 6882-11-12.

Cui, J., Yang, X., Dong, A., Cheng, D., Wang, J., Zhao, H., Xu, R., Wang, P. & Li, W. (2011). Chemical composition and antioxidant activity of Euphorbia fischeriana essential oil from China. Journal of Medicinal Plants Research, 5(19), 4794-4798.

Devasagayam, T.P.A., Tilak, J.C., Boloor, K.K., Sane, K.S., Ghaskadbi, S.S., & Lele, R.D. (2004). Free radicals and antioxidants in human health: current status and future prospects. The Journal of the Association of Physicians of India, 52, 794-804.

Ghasemzadeh,A., Azarifar, M., Soroodi, O. & Jaafar, Z.E. (2012). Flavonoid Compounds and their antioxidant activity in extract of some tropical plants. Journal of Medicinal Plants Research, 6(13), 2639-2643. doi: 10.5897/JMPR11.1531.

Goyal, A.K., Middha, S.K. & Sen, A. (2010). Evaluation of the DPPH radical scavenging activity, total phenols and antioxidant activities in Indian wild Bambusa vulgaris “Vittata” methanolic leaf extract. Journal of Natural Pharmaceuticals, 1(1), 40-45. doi: 10.4103/2229-5119.73586.

Gruyal, G.A. & del Rosario, R.M. (2013). Phytochemical profiles and quantifications of flavonoid contents of selected herbs in Cantilan, Surigao del Sur, Philippines. SDSSU Multidisciplinary Research Journal, 1(2), 126-133.

Hazra, B., Sarkar, R., Biswas, S. & Mandal, N. (2010). Comparative study of the antioxidant and reactive oxygen species scavenging properties in the extracts of the fruits of Terminalia chebula, Terminalia belerica and Emblica officinalis. BMC Complementary & Alternative Medicine, 10, 20. doi:10.1186/1472-6882-10-20.

Hatano, T., Edamatsu, R., Hiramatsu, M., Mori, A., Fujita, Y., Yasuhara, T., Yoshida, T. & Okuda, T. (1989). Effects of the interaction of tannins with co-existing substances VI: effects of tannins and related polyphenols on superoxide anion radical and on 1,1-diphenyl-2-picrylhydrazyl radical. Chemical and Pharmaceutical Bulletin, 37(8), 2016–2021. doi: 10.1248/cpb.37.2016

Oladunmoye, M. K. (2012). Antioxidant, free radical scavenging capacity and antimicrobial activities of Mirabilis jalapa. Journal of Medicinal Plants Research, 6(15), 2909-2913. doi: 10.5897/ JMPR09.281

Otang, W.M., Grierson, D.S. & Ndip, R. (2012). Phytochemical studies and antioxidant activity of two South African medicinal plants traditionally used for the management of opportunistic fungal infections in HIV/AIDS patients. BMC Complementary & Alternative Medicine, 12, 43. doi:10.1186/1472-6882-12-43

Ozkok, A., D’arcy, B.R. & Sorkun, K. (2010). Total phenolic acid and total flavonoid content of turkish pine honeydew honey. Journal of ApiProduct and ApiMedical Science, 2(2), 65-71. doi: 10.3896/ IBRA.4.02.2.01.

Philippine Herbal Medicine. (n.d.). List of Medicinal Plants in the Philippines. Retrieved from http://www. philippineherbalmedicine.org/medicinal_plants. htm.

Pourmorad, F., Hosseinimehr, S.J. & Shahabimajd, N. (2006). Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. African Journal of Biotechnology, 5(11), 1142-1145. Retrieved from http://www.academicjournals.org/ article/article1379770522_Pourmorad%20et%20 al.pdf.

Raghavendra, M., Reddy, A.M., Yadav, P.R., Raju, A.S. & Kumar, L.S. (2013). Comparative studies on the in vitro antioxidant properties of methanolic leafy extracts from six edible leafy vegetables of India. Asian Journal of Pharmaceutical and Clinical Research, 6(3), 96-99.

Ravipati, A.S., Zhang, L., Koyyalamudi, S.R., Jeong, S.C., Reddy, N., Bartlett, J., Smith, P.T., Shanmugam, K., Munch, G., Wu, M.J., Satyanarayanan, M. & Vysetti, B.(2012). Antioxidant and anti-inflammatory activities of selected Chinese medicinal plants and their relation with antioxidant content. BMC Complementary and Alternative Medicine,12, 173.

doi:10.1186/1472-6882-12-173.

Razali, N., Razab, R., Mat Junit, S. & Aziz, A. (2008). Radical scavenging and reducing properties of extracts of cashew shoots (Anacardium occidentale). Food Chemistry, 111(1), 38-44. doi:10.1016/j.foodchem.2008.03.024.

Saeed, N., Khan, M.R., & Shabbir, M. (2012). Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. BMC Complementary & Alternative Medicine, 12, 221. doi:10.1186/1472-6882-12-221.

Sasikumar, J.M., Mathew, G.M. & Darsini, D.T.P. (2010). Comparative Studies on Antioxidant Activity of methanol extract and flavonoid fraction of Nyctanthes arbor-tristis leaves. Electronic Journal of Environmental, Agriculture and Food Chemistry, 9(1), 227-233. Retrieved from http:// www.cabdirect.org/abstracts/20103066382. html;jsessionid.

Sellappan, S. & Akoh, C.C. (2002). Flavonoids and antioxidant capacity of Georgia-grown Vidalia onions. Journal of Agriculture and Food Chemistry, 50(9), 5338-5342.

Singh, R., Singh, S., Kumar, S. & Arora, S. (2007). Evaluation of antioxidant potential of ethyl acetate extract/fractions of Acacia auriculiformis A. Cunn. Food and Chemical Toxicology, 45(7), 1216–1223.

Shahidi, F., Janitha, P.K. & Wanasundara, P.D. (1992). Phenolic antioxidants. Critical reviews in Food Science and Nutrition, 32(1), 67-103. doi:10.1080/10408399209527581.

Pidugu, S. & Arun T. (2012). Antibacterial activity and phytochemical screening of Mentha arvensis Linn. against Proteus mirabilis from urinary tract infected patients. International Journal of PharmTech Research, 4(4), 1735- 1744.

Tawaha, K., Alali, F.Q., Gharaibeh, M., Mohammad, M. & El-Elimat, T. (2007). Antioxidant activity and total phenolic content of selected Jordanian plant species. Food Chemistry, 104(4), 1372–1378. doi:10.1016/j.foodchem.2007.01.064.

Tosun, M., Ercisli, S., Sengul, M., Ozer, H., Polat, T. & Ozturk, E. (2009). Antioxidant properties and total phenolic content of eight Salvia species from Turkey. Biological Research, 42(2), 175-181. doi: 10.4067/S0716-97602009000200005.


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