In vitro Evaluation of Different Elicitors against Fusarium Wilt of Musa textilis Nee (Abaca) caused by Fusarium oxysporum f. sp. cubense

Elizabeth P. Parac, Ruben Gapasin


Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc) is one of the most important diseases of abaca. The induction of systemic acquired resistance (SAR) in abaca plants offers a long lasting and sustainable approach in managing this disease. Pure culture of Foc isolates is inoculated into the 3-month old tissue cultured plants for pathogenicity test. Different elicitors
are tested in vitro and in vivo against Foc for induction of SAR in abaca. This study was conducted in order to: evaluate the antifungal property of different potential elicitors of resistance against abaca wilt disease in vitro and determine the efficacy of each elicitor for inducing SAR in abaca for the control of abaca wilt disease. The elicitors used such as irradiated chitosan (shrimp), nonirradiated chitosan (shrimp and crabs), Salicylic acid (SA), Silicon dioxide (SiO2), G-aminobutyric acid (GABA), and Benzothiadiazole (BTH Boost) have no antifungal property against the Foc. Both the phosphonate and benlate treatments have antifungal activity in vitro.The use of these elictors to induce SAR in abaca may be used as an alternative to the use of fungicides in
Fusarium wilt disease.


anti-fungal property, Fusarium oxysporum f. sp. cubense, Fusarium wilt disease, systemic acquired resistance (SAR)

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Bajet, N.B. & Magnaye, L.V. (2002). Virus diseases of banana and abaca in the Philippines. Los Banos, Laguna. PARFFI. 82 pp.

Boller, T. & Felix, G. (2009). A renaissance of elicitors: Perception of microbe-associated molecular patterns and danger signals by pattern-recognition receptors. Annu. Rev. Plant. Biol. 60:379-406. doi:10.1146/annurev.arplant.57.032905.105346.

Borines, L.M., Salamat, E.E. & Cardines, R.B. (2007). Reaction of abaca (Musa textilis Nee) germplasm to Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (E.F. Smith) Snyd. and Hans. Annals of Tropical Research, 29(1): 43-62.

Coffey, M.D. & Joseph, M.C. (1985). Effects of phosphorous acid and fosetyl-Al on the life cycle of Phytophthora cinnamomi and P. citricola. Phytopathology, 75:1042-1046.

Coffey, M.D. & Ouimette, D.G. (1989). Phosphonates: antifungal compounds against Oomycetes. In: nitrogen, phosphorous and sulphur utilization by fungi. (Eds. L. Boddy, R. Marchant and D.J. Read). Cambridge University Press. pp. 107-129.

Cohen, Y. & Coffey, M.D. (1986). Systemic fungicides and the control of the Oomycetes. Annual Review of Phytopathology, 24: 311-338. doi:

Davis, A.J., Say, M., Snow, A.J. & Grant, B.R. (1994).Sensitivity of Fusarium oxysporum f.sp. cubense to phosphonate. Plant Pathology, 43 (1): 200-205. doi: 10.1111/j.1365-3059.1994.tb00571.x.

Elmer, P.A.G. & Reglinski, T. (2006). Biosuppression of Botrytis cinerea in grapes. Plant Pathology, 55 (2): 155-177. doi: 10.1111/j.1365-3059.2006.01348.x.

Farih, A., Tsao, P.H. & Menge, J.A. (1981). Fungitoxic activity of efosite aluminum on growth, sporulation and germination of Phytophthora parasitica and P. citrophthora. Phytopathology, 71: 934- 936. doi:10.1094/Phyto-71-934.

Gorlach, J., Volrath, S., Knauf-Beiter, G., Hengy, G., Beckhove, U., Kogel, K.H., Oostendorp, M., Staub, T., Ward, E., Kessman, H. & Ryals, J. (1996). Benzothiadiazole, a novel class of inducers of SAR, activates gene expression and disease resistance in wheat. Plant Cell, 8(4): 629-643. doi: 10.1105/tpc.8.4.629.

IDCA. (1977). The abaca. UP, Los Banos, Laguna 3720 Philippines. 125pp.

Jailloux, F., Bugaret, Y. & Froidefond, G. (1987). Inhibition of sporulation of Phomopsis viticola Sacc. cause of dead arm disease of vines, by fosetyl-Al under field conditions. Crop Protection 6 (3): 148-152. doi:

Lichter, A., Gabler, F.M. & Smilanick, J.L. (2006). Control of spoilage in table grapes. Stewart Postharvest Review, 6 (1): 1-10. doi: 10.2212/spr.2006.6.1.

Magarey, P.A., Wachtel, M.F. & Newton, M.R. (1991). Evaluation of phosphonate, fosetyl-A1 and several phenylamide fungicides for postinfection control of grapevine downy mildew caused by Plasmopara viticola. Australasian Plant Pathology 20(2): 34-40. doi:https://doi.


NiƱo, M.J.C. (2009). Pathogenicity, characterization, identification of a bacterium causing heart rot disease of abaca and its control through elicitors of resistance. Undergraduate thesis manuscript. Visayas State University. 80 pp.

PCAARRD. (2003). Abaca industry status. Available at

Piamonte, R.T. (2011). Antifungal property of chemical elicitors and induction of systemic acquired resistance (SAR) for the control of rice blast caused by Pyricularia grisea Sacc. Unpublished M.S. thesis. Visayas State University. 89 pp.

Viranyi, F. & Oros, G. (1991). Developmental stage response to fungicides of Plasmopara halstedii (sunflower downy mildew). Mycological Research, 95(2):199-205. doi:

Wicks, T.J. & Hall, B. (1988). Preliminary evaluation of phosphorus acid, fosetyl-Al- and metalaxyl for controlling Phytophthora cambivora on almond and cherry. Crop Protection, 7(5): 314-318. doi:

Williams, D.J., Beach, B.G.W., Horriere, D. & Marechal, G. (1977). LS 74-783, a new systemic fungicide with activity against Phycomycete diseases. Proceedings of the 1977 British Crop Protection Conference Pests and Diseases, pp.565-573.


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